دوره 1، شماره 1 - ( 9-1403 )
جلد 1 شماره 1 صفحات 14-9 |
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Khatibi L, Akbarzadeh K, Rassi Y, Baniardalan M, Akbari M, Shavali Z, et al . Effect of Sterilization on Lucilia sericata (Diptera: Calliphoridae) Eggs and larval stage in laboratory condition. Journal of Health Sciences Perspective 2024; 1 (1) :9-14
URL: http://jhsp.medilam.ac.ir/article-1-22-fa.html
URL: http://jhsp.medilam.ac.ir/article-1-22-fa.html
Effect of Sterilization on Lucilia sericata (Diptera: Calliphoridae) Eggs and larval stage in laboratory condition. Journal of Health Sciences Perspective. 1403; 1 (1) :9-14
چکیده: (67 مشاهده)
Introduction: Maggot debridement therapy (also known as larval therapy) is a type of biotherapy that involves the use of live, sterile maggots (flies’ larvae) to clean up non-healing and soft-tissue wounds, treat the necrotic (dead) and sloughy tissue. This method represents a controlled myiasis in which necrophagous fly larvae will clean the wound and stimulate growth hormones, cell reproduction, and the body’s immune system. This group of flies, commonly known as necrophagous flies and have no harmful effects on healthy tissue, is the most appropriate choice. This research was conducted to investigate the effect of being sterile or non-sterile on the hatching rate of eggs and the persistence of the larval stage (1st instar) of Lucilia sericata.
Materials & Methods: A sweep net and bottle fly traps (reverse-cone model) were used to collect flies and identified by using valid systematic keys. The L. sericata eggs were placed separately in 2 and 3L-lidded plastic containers with some meat. With the emergence of third instar larvae, they were transferred to sawdust containers and then into 40x40x40 cages containing sugar or honey dissolved in water (5% solution), to transform into pupae and adults. To determine the shelf life of L. sericata eggs and larvae at refrigerator temperature, they were divided into sterile and non-sterile (without intervention) groups.
Results: Out of 5400 eggs, including 2700 sterile eggs and 2700 normal eggs, the highest percentage of eggs hatching referred to the sterile group (73%). However, there is no significant difference between the groups. The results of the study on the effects of sterility or non-sterility on the growth and development of the 1st instar larvae showed that no significant difference was observed between the two groups. The best time for eggs to hatch and their transformation into 1st instar was 72 hours and the best survival time of larvae was in 24 hours. The results obtained from this graph show that the most suitable temperature for eggs to hatch is 6°C and the most suitable temperature for survival of 1st instar larvae are 2 and 6 °C.
Conclusion: The results of this study showed, that the sterility or non-sterility of eggs and larvae cannot affect their durability.
Materials & Methods: A sweep net and bottle fly traps (reverse-cone model) were used to collect flies and identified by using valid systematic keys. The L. sericata eggs were placed separately in 2 and 3L-lidded plastic containers with some meat. With the emergence of third instar larvae, they were transferred to sawdust containers and then into 40x40x40 cages containing sugar or honey dissolved in water (5% solution), to transform into pupae and adults. To determine the shelf life of L. sericata eggs and larvae at refrigerator temperature, they were divided into sterile and non-sterile (without intervention) groups.
Results: Out of 5400 eggs, including 2700 sterile eggs and 2700 normal eggs, the highest percentage of eggs hatching referred to the sterile group (73%). However, there is no significant difference between the groups. The results of the study on the effects of sterility or non-sterility on the growth and development of the 1st instar larvae showed that no significant difference was observed between the two groups. The best time for eggs to hatch and their transformation into 1st instar was 72 hours and the best survival time of larvae was in 24 hours. The results obtained from this graph show that the most suitable temperature for eggs to hatch is 6°C and the most suitable temperature for survival of 1st instar larvae are 2 and 6 °C.
Conclusion: The results of this study showed, that the sterility or non-sterility of eggs and larvae cannot affect their durability.
نوع مطالعه: پژوهشي |
موضوع مقاله:
بهداشت عمومی
دریافت: 1403/6/11 | پذیرش: 1403/8/11 | انتشار: 1403/10/7
دریافت: 1403/6/11 | پذیرش: 1403/8/11 | انتشار: 1403/10/7
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